細胞の個性とことばを読み解いて、
生命と疾患の本質を理解する。

「シグナル伝達」「データサイエンス」「オミクス解析」を融合し、がん転移・休眠・不均一性の分子メカニズムを解明します。
「どのようながん細胞」が「どの臓器」に「どの遺伝子」を利用して転移し、「どのような細胞」と「どのような情報交換」を行なっているのかを理解し、生体内の細胞情報交換を包括的に理解することを目指します。
そのために、WET実験とDRY解析を組み合わせた独自の研究体制を構築しています。

論文・総説・書籍

* = Corresponding Author / † = Contributed Equally

Articles & Reviews

1. Akter-Yasuoka E†, Lee T†, Murayama T*, Nakayama J*. Context-Dependent cGAS-STING Activation Shapes Metastatic Progression and Dormancy. Cells. 2026; 15(17):1619. Review *Corresponding Author PubMed
2. Urabe F*, Yoshihara K, Nakayama J, Hirano Y, Watanabe N, Suzuki H, Umemori M, Ito K, Igarashi T, Sato S, Kimura T, Furata A, Yamamoto Y*. Single Cell Transcriptomics Reveals Bladder Microenvironment Dynamics in Hunner Type Interstitial Cystitis. iScience. 2026 Jul 9;29(8):116725. PubMed
3. Shoji T, Shinojima A, Kishimoto T, Sato K, Ikegami N, Yumoto E, Shindo R, Uchida Y, Kusumi S, Koga D, Yamamoto E, Hirano Y, Ogino R, Shibata H, Izawa K, Yasumi T, Sato R, Nakayama J, Higashiyama S, Hasegawa J, Kajiho H, Sasaki T, Kuchitsu Y*, Taguchi T*. CHMP4B is a lysosomal effector of PI(3,5)P2, facilitating ESCRT-mediated microautophagic degradation of STING. Nature Communications. 2026 May 27;17(1). PubMed
4. Tokura M, Nakayama J, Suzuki H, Ochi M, Umemori M, Shiino S, Yoshida M, Takayama S, Yatabe Y, Yamamoto Y*. Spatial Transcriptomics and bulk RNA-seq analysis revealed Molecular Classification of Invasive Lobular Carcinoma. Cancer Science. 2026 May 21;. PubMed
5. Tsubaki S, Nashimoto S, Tanaka R, Tsutsuki H, Kato K, Nakayama J, Kimura K, Oshima K, Yamamoto Y, Okusaka T, Esaki M, Kawai N, Ota N, Yoshioka Y, Ochiya T, Saito Y, Sawa T, Hozumi K, Matsuzaki J*, Tsugawa H*. Gut commensal Klebsiella pneumoniae extracellular vesicles shape a liver microenvironment conducive to gut-liver bacterial translocation and pro-tumorigenic processes. Journal of Extracellular Vesicles. 2026 Apr;15(4):e70262. PubMed
6. Hirano Y, Suzuki H, Nakayama J, Yamamoto T, Hirata E, Araya J, Fujita Y*, Yamamoto Y*. An integrated single-cell lung cancer atlas reveals distinct fibroblast phenotypes between adenocarcinoma and squamous cell carcinomas. npj Precision Oncology. 2026 Jan 23;10(1):72. PubMed
7. Vu DA†, Mori S†, Akamatsu K, Nakayama J, Sakamoto T*. Ring-finger protein 126 (RNF126) promotes anoikis resistance and peritoneal dissemination in ovarian cancer. International Journal of Molecular Sciences. 2025;26(24):12183. PubMed
8. Azuma K, Matsuyama T, Watanabe S, Semba K*, Nakayama J*. TIE1 promotes primary tumor growth by inhibiting apoptosis and activating AKT-p70S6K signaling pathway in breast cancer. Genes to Cells. 2025 Nov;30(6):e70062. *Corresponding Author PubMed
9. Habu K, Matsuoka Y, Hiyoshi H, Nakayama J, Watanabe K, Tate S, Sakaue T, Murai J, Uno K, Nishie H, Kubota E, Kataoka H, Joh T, Watanabe Y, Oshikiri T, Higashiyama S*. KLHL5 Contributes to Colorectal Cancer Cell Survival by Promoting Cell Cycle Progression and Suppressing Apoptotic Cell Death. Cancer Science. 2025 Sep;116(9):2444–2456. PubMed
10. Uno K, Nishie H, Hiyoshi H, Habu K, Nakayama J, Tate S, Sakaue T, Kubota E*, Tanaka M, Shimura T, Kataoka H, Joh T, Higashiyama S. Implication of KLHL Gene Family Member KLHL5 in Colorectal Cancer Progression and Prognosis. Cancer Science. 2025 Sep;116(9):2580–2591. PubMed
11. Koide S†, Yumoto E†, Nakayama J, Higashiyama S, Kuchitsu Y*, Taguchi T*. Cell biological insights into human STING variants. Cell Structure and Function. 2025 Jun 7;50(1):135–144. Review PubMed
12. Yoshida K, Yokoi A*, Suzuki H, Tamauchi S, Kitagawa M, Inami E, Nakayama J, Mori Y, Okamoto K, Suzuki Y, Yoshida H, Kato T, Kajiyama H, Yamamoto Y*. Single-Nucleus RNA Sequencing and Spatial Transcriptomics for Squamous Cell Carcinoma Arising From Ovarian Mature Teratoma. Cancer Science. 2025 May;116(5):1339–1351. PubMed
13. Weisheng Z, Nakayama J, Inomata Y, Higashiyama S, Hiratsuka T*. A sensitive ERK fluorescent probe reveals the significance of minimal EGF-induced transcription. Cell Structure and Function. 2025 Feb 7;50(1):15–24. PubMed
14. Nakamichi K, Suzuki H, Yamamoto Y, Semba K, Nakayama J*. Nuclear receptor profiling for subtype classification and as prognostic markers in 33 cancer types. Discover Oncology. 2024 Dec 24;15(1):834. *Corresponding Author PubMed
15. Murayama T, Mahadevan NR, Meador CB, Ivanova EV, Pan Y, Knelson EH, Tani T, Nakayama J, Ma X, Thai TC, Hung YP, Kim W, Watanabe H, Cai KQ, Hata AN, Paweletz CP, Barbie DA, Cañadas I*. Targeting TREX1 Induces Innate Immune Response in Drug-Resistant Small-Cell Lung Cancer. Cancer Research Communications. 2024 Sep 1;4(9):2399–2414. PubMed
16. Yamamoto T†, Nakayama J†, Urabe F, Ito K, Nishida-Aoki N, Kitagawa M, Yokoi A, Kuroda M, Hattori Y, Yamamoto Y, Ochiya T. Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progression. Cell Reports. 2024 Aug 27;43(8):114517. †Contributed Equally PubMed
17. Kuroiwa Y*, Ito K, Nakayama J, Semba K, Yamamoto Y*. Analysis of the responsiveness to antiandrogens in multiple breast cancer cell lines. Genes to Cells. 2024 Apr;29(4):301–315. PubMed
18. Murayama T, Nakayama J, Jiang X, Miyata K, Morris AD, Cai KQ, Prasad RM, Ma X, Efimov A, Belani N, Gerstein ER, Tan Y, Zhou Y, Kim W, Maruyama R, Campbell KS, Chen L, Yang Y, Balachandran S, Cañadas I*. Targeting DHX9 Triggers Tumor-Intrinsic Interferon Response and Replication Stress in Small Cell Lung Cancer. Cancer Discovery. 2024 Mar 1;14(3):468–491. PubMed
19. Ishibashi K, Ichinose T, Kadokawa R, Mizutani R, Iwabuchi S, Togi S, Ura H, Tange S, Shinjo K, Nakayama J, Nanjo S, Niida Y, Kondo Y, Hashimoto S, Sahai E, Yano S, Nakada M, Hirata E*. Astrocyte-induced mGluR1 activates human lung cancer brain metastasis via glutamate-dependent stabilization of EGFR. Developmental Cell. 2024 Mar 11;59(5):579–594.e6. PubMed
20. Nakamichi K, Yamamoto Y, Semba K*, Nakayama J*. Metastatic potentials classified with hypoxia-inducible factor 1 downstream genes in pan-cancer cell lines. Genes to Cells. 2024 Feb;29(2):169–177. *Corresponding Author PubMed
21. Yamaguchi K†, Nakayama J†*, Yamamoto T†, Semba K, Shirota T, Yamamoto Y*. Collagen induction of immune cells in the mammary glands during pregnancy. Physiological Genomics. 2024 Feb 1;56(2):128–135. *Corresponding Author, †Contributed Equally APS Select Award in Physiological Genomics PubMed
22. Inoue C, Mukai K, Matsudaira T, Nakayama J, Kono N, Aoki J, Arai H, Uchida Y*, Taguchi T*. PPP1R12A is a recycling endosomal phosphatase that facilitates YAP activation. Scientific Reports. 2023 Nov 13;13(1):19740. PubMed
23. Nakayama J*, Yamamoto Y*. Cancer-prone Phenotypes and Gene Expression Heterogeneity at Single-cell Resolution in Cigarette-smoking Lungs. Cancer Research Communications. 2023 Nov 10;3(11):2280–2291. *Corresponding Author PubMed
24. Yokoi A*, Yoshida K, Koga H, Kitagawa M, Nagao Y, Iida M, Kawaguchi S, Zhang M, Nakayama J, Yamamoto Y, Baba Y, Kajiyama H, Yasui T*. Spatial exosome analysis using cellulose nanofiber sheets reveals the location heterogeneity of extracellular vesicles. Nature Communications. 2023 Nov 8;14(1):6915. PubMed
25. Ito K, Yamamoto T, Hayashi Y, Sato S, Nakayama J, Urabe F, Shimasaki T, Nakamura E, Matui Y, Fujimoto H, Kimura T, Egawa S, Ochiya T*, Yamamoto Y*. Osteoblast-derived extracellular vesicles exert osteoblastic and tumor-suppressive functions via SERPINA3 and LCN2 in prostate cancer. Molecular Oncology. 2023 Oct;17(10):2147–2167. PubMed
26. Shiino S*, Tokura M, Nakayama J, Yoshida M, Suto A, Yamamoto Y*. Investigation of Tumor Heterogeneity Using Integrated Single-Cell RNA Sequence Analysis to Focus on Genes Related to Breast Cancer-, EMT-, CSC-, and Metastasis-Related Markers in Patients with HER2-Positive Breast Cancer. Cells. 2023 Sep 15;12(18). PubMed
27. Han Y*, Katayama S, Futakuchi M, Nakamichi K, Wakabayashi Y, Sakamoto M, Nakayama J*, Semba K*. Targeting c-Jun Is a Potential Therapy for Luminal Breast Cancer Bone Metastasis. Molecular Cancer Research. 2023 Sep 1;21(9):908–921. *Corresponding Author PubMed
28. Yoshida K, Yokoi A*, Kitagawa M, Sugiyama M, Yamamoto T, Nakayama J, Yoshida H, Kato T, Kajiyama H, Yamamoto Y*. Downregulation of miR-10b-5p facilitates the proliferation of uterine leiomyosarcoma cells: A microRNA sequencing-based approach. Oncology Reports. 2023 May;49(5). PubMed
29. Hayashi Y, Nakayama J*, Yamamoto M, Maekawa M, Watanabe S, Higashiyama S, Inoue JI, Yamamoto Y, Semba K*. Aberrant accumulation of NIK promotes tumor growth by dysregulating translation and post-translational modifications in breast cancer. Cancer Cell International. 2023 Apr 1;23(1):57. *Corresponding Author PubMed
30. Azuma K, Sakamoto M, Katayama S, Matsui A, Nakamichi K, Goshima N, Watanabe S, Nakayama J*, Semba K*. HOXB7 induces STAT3-mediated transformation and lung metastasis in immortalized mammary gland NMuMG cells. Genes to Cells. 2023 Apr;28(4):277–287. PubMed
31. Watanabe N†, Fujita Y†*, Nakayama J†, Mori Y, Kadota T, Hayashi Y, Shimomura I, Ohtsuka T, Okamoto K, Araya J, Kuwano K, Yamamoto Y*. Anomalous Epithelial Variations and Ectopic Inflammatory Response in Chronic Obstructive Pulmonary Disease. American Journal of Respiratory Cell and Molecular Biology. 2022 Dec;67(6):708–719. †Contributed Equally PubMed
32. Tokura M†, Nakayama J†, Prieto-Vila M, Shiino S, Yoshida M, Yamamoto T, Watanabe N, Takayama S, Suzuki Y, Okamoto K, Ochiya T, Kohno T, Yatabe Y, Suto A, Yamamoto Y*. Single-Cell Transcriptome Profiling Reveals Intratumoral Heterogeneity and Molecular Features of Ductal Carcinoma In Situ. Cancer Research. 2022 Sep 16;82(18):3236–3248. †Contributed Equally PubMed
33. Nakayama J*, Matsunaga H, Arikawa K, Yoda T, Hosokawa M, Takeyama H, Yamamoto Y, Semba K*. Identification of two cancer stem cell-like populations in triple-negative breast cancer xenografts. Disease Models & Mechanisms. 2022 Jun 1;15(6). *Corresponding Author PubMed
34. Han Y, Azuma K, Watanabe S, Semba K, Nakayama J*. Metastatic profiling of HER2-positive breast cancer cell lines in xenograft models. Clinical & Experimental Metastasis. 2022 Jun;39(3):467–477. *Corresponding Author PubMed
35. Yoshida K, Yokoi A*, Yamamoto T, Hayashi Y, Nakayama J, Yokoi T, Yoshida H, Kato T, Kajiyama H, Yamamoto Y*. Aberrant Activation of Cell-Cycle-Related Kinases and the Potential Therapeutic Impact of PLK1 or CHEK1 Inhibition in Uterine Leiomyosarcoma. Clinical Cancer Research. 2022 May 13;28(10):2147–2159. PubMed
36. Yamamoto T, Nakayama J, Yamamoto Y, Kuroda M, Hattori Y*, Ochiya T*. SORT1/LAMP2-mediated extracellular vesicle secretion and cell adhesion are linked to lenalidomide resistance in multiple myeloma. Blood Advances. 2022 Apr 26;6(8):2480–2495. PubMed
37. Ayukawa S, Kamoshita N†, Nakayama J†, Teramoto R, Pishesha N, Ohba K, Sato N, Kozawa K, Abe H, Semba K, Goda N, Fujita Y*, Maruyama T*. Epithelial cells remove precancerous cells by cell competition via MHC class I-LILRB3 interaction. Nature Immunology. 2021 Nov;22(11):1391–1402. †Contributed Equally PubMed
38. Yogosawa S, Ohkido M, Horii T, Okazaki Y, Nakayama J, Yoshida S, Toyokuni S, Hatada I, Morimoto M, Yoshida K*. Mice lacking DYRK2 exhibit congenital malformations with lung hypoplasia and altered Foxf1 expression gradient. Communications Biology. 2021 Oct 20;4(1):1204. PubMed
39. Nishiyama K, Maekawa M*, Nakagita T, Nakayama J, Kiyoi T, Chosei M, Murakami A, Kamei Y, Takeda H, Takada Y, Higashiyama S*. CNKSR1 serves as a scaffold to activate an EGFR phosphatase via exclusive interaction with RhoB-GTP. Life Science Alliance. 2021 Sep;4(9). PubMed
40. Nakayama J*, Han Y, Kuroiwa Y, Azuma K, Yamamoto Y, Semba K. The In Vivo Selection Method in Breast Cancer Metastasis. International Journal of Molecular Sciences. 2021 Feb 14;22(4). Review *Corresponding Author PubMed
41. Saito-Moriya R†*, Nakayama J†, Kamiya G, Kitada N, Obata R, Maki SA, Aoyama H. How to Select Firefly Luciferin Analogues for In Vivo Imaging. International Journal of Molecular Sciences. 2021 Feb 12;22(4). Review †Contributed Equally PubMed
42. Yogosawa S, Nakayama J, Nishi M, Ryo A, Yoshida K*. Carbonic anhydrase 13 suppresses bone metastasis in breast cancer. Cancer Treatment and Research Communications. 2021;27:100332. PubMed
43. Nakayama J†*, Saito R†, Hayashi Y, Kitada N, Tamaki S, Han Y, Semba K, Maki SA. High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai. International Journal of Molecular Sciences. 2020 Oct 24;21(21). *Corresponding Author PubMed
44. Kuroiwa Y, Nakayama J*, Adachi C, Inoue T, Watanabe S, Semba K. Proliferative Classification of Intracranially Injected HER2-positive Breast Cancer Cell Lines. Cancers. 2020 Jul 6;12(7). *Corresponding Author PubMed
45. Han Y†, Nakayama J†*, Hayashi Y, Jeong S, Futakuchi M, Ito E, Watanabe S, Semba K. Establishment and characterization of highly osteolytic luminal breast cancer cell lines by intracaudal arterial injection. Genes to Cells. 2020 Feb;25(2):111–123. *Corresponding Author, †Contributed Equally Top Cited Article Award 2020–2021 PubMed
46. Tanaka M*, Shiota M, Koyama M, Nakayama J, Yashiro M, Semba K, Goda N. Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 2020 Feb;39(1):23–26. PubMed
47. Maekawa M*, Hiyoshi H, Nakayama J, Kido K, Sawasaki T, Semba K, Kubota E, Joh T, Higashiyama S*. Cullin-3/KCTD10 complex is essential for K27-polyubiquitination of EIF3D in human hepatocellular carcinoma HepG2 cells. Biochemical and Biophysical Research Communications. 2019 Sep 3;516(4):1116–1122. PubMed
48. Tanigawa K, Maekawa M*, Kiyoi T, Nakayama J, Kitazawa R, Kitazawa S, Semba K, Taguchi T, Akita S, Yoshida M, Ishimaru K, Watanabe Y, Higashiyama S*. SNX9 determines the surface levels of integrin β1 in vascular endothelial cells: Implication in poor prognosis of human colorectal cancers overexpressing SNX9. Journal of Cellular Physiology. 2019 Aug;234(10):17280–17294. PubMed
49. Murakami A, Maekawa M*, Kawai K, Nakayama J, Araki N, Semba K, Taguchi T, Kamei Y, Takada Y, Higashiyama S*. Cullin-3/KCTD10 E3 complex is essential for Rac1 activation through RhoB degradation in human epidermal growth factor receptor 2-positive breast cancer cells. Cancer Science. 2019 Feb;110(2):650–661. PubMed
50. Matsudaira T, Mukai K, Noguchi T, Hasegawa J, Hatta T, Iemura SI, Natsume T, Miyamura N, Nishina H, Nakayama J, Semba K, Tomita T, Murata S, Arai H, Taguchi T*. Endosomal phosphatidylserine is critical for the YAP signalling pathway in proliferating cells. Nature Communications. 2017 Nov 1;8(1):1246. PubMed
51. Nakayama J, Ito E, Fujimoto J, Watanabe S, Semba K*. Comparative analysis of gene regulatory networks of highly metastatic breast cancer cells established by orthotopic transplantation and intra-circulation injection. International Journal of Oncology. 2017 Feb;50(2):497–504. PubMed

書籍

1. 実験医学別冊 論文に出る遺伝子 デルジーン300. 中山 淳（分担執筆）, 羊土社, 2024年12月. ISBN: 9784758122771.
2. 実験医学別冊 RNA-Seqデータ解析 WETラボのための超鉄板レシピ. 中山 淳, 山本 雄介（分担執筆, コラム バルク解析とシングルセル解析）, 羊土社, 2023年10月. ISBN: 9784758122672.

関連リンク

所属機関

共同研究機関

研究会・学会